Explore: Sephadex

Sephadex is a cross-linked dextran gel used for gel filtration. It was launched by Pharmacia in 1959, after development work by Jerker Porath and Per Flodin

(kDa) 1 Sephadex G-10 0-0.7 2 Sephadex G-25 1-5 3 Sephadex G-50 1.5-30 4 Sephadex G-75 3-70 5 Sephadex G-100 4-150 6 Sephadex G-150 5-300 7 Sephadex G-200

the negative charge of the substance. A slurry of resin, such as DEAE-Sephadex is poured into the column. The matrix that is used is insoluble with charged

Pregnenolone product can be separated from cholesterol substrate using Sephadex LH-20 minicolumns. Pregnenolone is lipophilic and readily crosses the blood–brain

from a positive side group of diethylaminoethyl bound to cellulose or Sephadex. Agarose gel based medium contain large pores as well but their substitution

Monolayer of cell must be grown. Moreover, plastic beads of polystyrene, sephadex and polyacrylamide are also available for cell growth in suspension culture

of California, Berkeley Thesis Fraktionierungen von Proteinen – besonders Enzymen – mit Ionenaustauschern und Molekularsieben <vom Typ Sephadex> (1963)

purify cholesterol prior to use. Cholesterol can be purified using small Sephadex LH-20 columns. Cholesterol is oxidized by the liver into a variety of bile

Styragel (cross-linked polystyrene-divinylbenzene), LH-20 (hydroxypropylated Sephadex), Bio-Gel (cross-linked polyacrylamide), HW-20 & HW-40 (hydroxylated methacrylic

head of research. In 1959, Pharmacia pioneered gel filtration with its Sephadex products. These were also based on dextran and discoveries in Tiselius'