Explore: Insertional Mutagenesis

molecular biology, insertional mutagenesis is the creation of mutations in DNA by the addition of one or more base pairs. Such insertional mutations can occur

Directed mutagenesis Site-directed mutagenesis/ PCR mutagenesis Insertional mutagenesis Signature tagged mutagenesis Transposon mutagenesis Sequence saturation

mutagenesis is not. Other techniques of mutagenesis include combinatorial and insertional mutagenesis. Mutagenesis that is not random can be used to clone

occurring mutations or inducing mutants with radiation, chemicals, or insertional mutagenesis (e.g. transposable elements). Subsequent breeding takes place,

colleagues set out to develop a large-scale insertional mutagenesis method. Although large-scale chemical mutagenesis screens were getting underway in several

size of the insertion, multiple new amino acids that may affect the function of the protein.[citation needed] Indel Insertional mutagenesis Loss-of-Function

vectors included strong viral promoters which had a side effect of insertional mutagenesis, nuclear DNA mutations that affect the function of a gene. These

element has found wide use in Drosophila research as a mutagen. The mutagenesis system typically uses an autonomous but immobile element, and a mobile

Transposon insertion sequencing (Tn-seq) combines transposon insertional mutagenesis with massively parallel sequencing (MPS) of the transposon insertion sites

Chen W, Sun Z, Townsend K, et al. (October 1999). "A large-scale insertional mutagenesis screen in zebrafish". Genes & Development. 13 (20): 2713–2724.