Explore: Artificial Nucleases

a technique known as genome editing with engineered nucleases. Alongside zinc finger nucleases and CRISPR/Cas9, TALEN is a prominent tool in the field

major classes of these enzymes—zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and engineered meganucleases—were

artificial restriction enzymes can target large DNA sites (up to 36 bp) and can be engineered to bind to desired DNA sequences. Zinc finger nucleases

application in the clinic. His research also advanced the "use of artificial nucleases for targeted genome editing in cell and gene therapy." He also serves

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger

for a specific sequence is an area of active research, and zinc finger nucleases and zinc finger transcription factors are two of the most important applications

(CRISPR)-Cas9, transcription activator-like effector nucleases (TALEN), meganucleases, and zinc finger nucleases (ZFN). These tools use different mechanisms to

finger nucleases. Since 2009 more accurate and easier systems to implement have been developed. Transcription activator-like effector nucleases (TALENs)

offer an editorial comment in the journal Gene: The work on restriction nucleases not only permits us easily to construct recombinant DNA molecules and

Nishino T, Morikawa K (December 2002). "Structure and function of nucleases in DNA repair: shape, grip and blade of the DNA scissors". Oncogene. 21