The complement system - Info and Reading Options

"The complement system" Table Of Contents:

• 1- Overview -- Mihaela Gadjeva
• 2- Evaluation of complement function by ELISA -- Anja Roos and Jörgen Wieslander
• 3- Classical complement pathway component C1q : purification of human C1q, isolation of C1q collagen-like and globular head fragments and production of recombinant C1q-derivatives. Functional characterization -- Mihaela Kojouharova
• 4- Classical complement pathway components C1r and C1s : purification from human serum and in recombinant form and functional characterization -- Véronique Rossi ... [et al.]
• 5- Expression and purification methods for the production of recombinant human complement component C2 -- Angela W. Norton ... [et al.]
• 6- Purification and characterization of human and mouse complement C3 -- Marieta M. Ruseva and Meike Heurich
• 7- Purification of human complement protein C5 -- Lars Sottrup-Jensen and Gregers Rom Andersen
• 8- Quantification of complement C5b-9 binding to cells by flow cytometry -- Oren Moskovich and Zvi Fishelson
• 9- Challenges and opportunities in fractionation of recombinant human mannan-binding lectin -- Thomas Vorup-Jensen
• 10- Genotyping of FCN and MBL2 polymorphisms using pyrosequencing -- Lea Munthe-Fog, Hans Ole Madsen, and Peter Garred
• 11- Assay for estimation of the functional activity of the mannan-binding lectin pathway of the complement system -- Troels R. Kjaer and Steffen Thiel
• 12- Purification, measurement of concentration, and functional complement assay of human ficolins -- Misao Matsushita ... [et al.]
• 13- Purification and functional analysis of human properdin -- Joseph O'Flynn ... [et al.]
• 14- Purification and functional characterization of C4b- binding protein (C4BP) -- Frida C. Mohlin and Anna M. Blom
• 15- Purification and functional characterization of factor I -- Sara C. Nilsson and Anna M. Blom
• 16- C1 inhibitor : quantification and purification -- Lilian Varga and József Dobó
• 17- Purification, quantification, and functional analysis of complement factor H -- Bing-Bin Yu ... [et al.]
• 18- Factor H-related proteins -- Mihály Józsi and Seppo Meri
• 19- Functional evaluation of factor H genetic and acquired abnormalities : application for atypical hemolytic uremic syndrome (aHUS) -- Lubka T. Roumenina ... [et al.]
• 20- Anti-factor H autoantibodies assay -- Marie-Agnès Dragon-Durey ... [et al.]
• 21- Purification of native surfactant protein SP-A from pooled amniotic fluid and bronchoalveolar lavage -- Najmunisa Karbani ... [et al.]
• 22- Purification of surfactant protein D (SP-D) from pooled amniotic fluid and bronchoalveolar lavage -- Eswari Dodagatta-Marri ... [et al.]
• 23- Functional analysis of C5a effector responses in vitro and in vivo -- Christian M. Karsten, Yves Laumonnier, and Jörg Köhl
• 24- Detection of complement receptors 1 and 2 on mouse splenic B cells using flow cytometry -- Luke R. Donius and John H. Weis
• 25- Quantification of complement receptor 2 calcium signaling enhancement using flow cytometry -- Luke R. Donius and John H. Weis
• 26- Purification of C1q receptors and functional analysis -- Berhane Ghebrehiwet and Ellinor I.B. Peerschke
• 27- Detection of cell membrane-bound CD46 using flow cytometry -- Martin Kolev and Claudia Kemper
• 28- Detection of CD59 using flow cytometry -- Martin Kolev
• 29- Functional analysis of CD59 using complement-dependent cytotoxicity assay -- Martin Kolev
• 30- Detection of soluble CR3 (CD11b/CD18) by time-resolved immunofluorometry -- Gitte Krogh Nielsen and Thomas Vorup-Jensen
• 31- Analysis of the complement activation in mice -- Nirmal K. Banda and Kazue Takahashi
• 32- Opsonophagocytic assay -- Markryan Dwyer and Mihaela Gadjeva.

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