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Posttranslational Modification Of Proteins by Christopher Walsh

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1Posttranslational Modification Of Differentially Expressed Mitochondrial Proteins In The Retina During Early Experimental Autoimmune Uveitis.

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This article is from Molecular Vision , volume 17 . Abstract Purpose: Posttranslational modification of proteins plays an important role in cellular functions and is a key event in signal transduction pathways leading to oxidative stress and DNA damage. In this study, we used matrix-assisted laser desorption/ionization- time of flight (MALDI-TOF) to investigate the posttranslational modifications of the differentially expressed proteins in the retinal mitochondria during early experimental autoimmune uveitis (EAU). Methods: EAU was induced in 18 B10RIII mice with 25 µg of inter-photoreceptor retinoid-binding protein (IRBP) emulsified with complete Freund’s adjuvant (CFA); 18 mice treated with CFA without IRBP served as controls. Retinas were removed from the experimental and control groups on day 7 post immunization; mitochondrial fractions were extracted and subjected to 2 dimentional-difference in gel electrophoresis (2D-DIGE); and the protein spots indicating differential expression were subjected to MALDI-TOF for protein identification and indication of any posttranslational modifications. Results: Of the 13 proteins found to be differentially expressed by 2D-DIGE (including upregulated aconitase, mitochondrial heat shock protein (mtHsp) 70, lamin-1, syntaxin-binding protein, αA crystallin, βB2 crystallin, along with downregulated guanine nucleotide-binding protein and ATP synthase) nine were found to undergo posttranslational modification. Oxidation was a common modification found to occur on aconitase, mtHsp 70, ATP synthase, lamin-1, βB2-crystallin, guanine nucleotide-binding protein, and manganese superoxide dismutase (MnSOD). In addition, aconitase hydratase, mtHsp 70, guanine nucleotide-binding protein, ATP synthase, syntaxin-binding protein, βB2-crystallin, and lamin-1 were also modified by carbamidomethylation. αA-crystallin had a pyro-glu modification. Conclusions: Several proteins present in the retinal mitochondria are posttranslationally modified during early EAU, indicating the presence of oxidative stress and mitochondrial DNA damage. The most common modifications are oxidation and carbamidomethylation. A better understanding of the proteins susceptible to posttranslational modifications in the mitochondria at the early stage of the disease may serve to advance therapeutic interventions to attenuate disease progression.

“Posttranslational Modification Of Differentially Expressed Mitochondrial Proteins In The Retina During Early Experimental Autoimmune Uveitis.” Metadata:

  • Title: ➤  Posttranslational Modification Of Differentially Expressed Mitochondrial Proteins In The Retina During Early Experimental Autoimmune Uveitis.
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  • Language: English

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The book is available for download in "texts" format, the size of the file-s is: 6.95 Mbs, the file-s for this book were downloaded 85 times, the file-s went public at Tue Oct 28 2014.

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2Chemical And Functional Aspects Of Posttranslational Modification Of Proteins.

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This article is from Acta Naturae , volume 1 . Abstract This paper reviews the chemical and functional aspects of the posttranslational modifications of proteins, which are achieved by the addition of various groups to the side chain of the amino acid residue backbone of proteins. It describes the main prosthetic groups and the interaction of these groups and the apoenzyme in the process of catalysis, using pyridoxal catalysis as an example. Much attention is paid to the role of posttranslational modification of proteins in the regulation of biochemical processes in live organisms, and especially to the role of protein kinases and their respective phosphotases. Methylation and acetylation reactions and their role in the "histone code", which regulates genome expression on the transcription level, are also reviewed. This paper also describes the modification of proteins by large hydrophobic residues and their role in the function of membrane-associated proteins. Much attention is paid to the glycosylation of proteins, which leads to the formation of glycoproteins. We also describe the main non-enzymatic protein modifications such as glycation, homocysteination, and desamida-tion of amide residues in dibasic acids.

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  • Title: ➤  Chemical And Functional Aspects Of Posttranslational Modification Of Proteins.
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  • Language: English

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3Characterization Of A Novel Posttranslational Modification In Polypyrimidine Tract-binding Proteins By SUMO1.

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This article is from BMB Reports , volume 47 . Abstract Polypyrimidine tract-binding protein 1 (PTBP1) and its brainspecific homologue, PTBP2, are associated with pre-mRNAs and influence pre-mRNA processing, as well as mRNA metabolism and transport. They play important roles in neural differentiation and glioma development. In our study, we detected the expression of the two proteins in glioma cells and predicted that they may be sumoylated using SUMOplot analyses. We confirmed that PTBP1 and PTBP2 can be modified by SUMO1 with co-immunoprecipitation experiments using 293ET cells transiently co-expressing SUMO1 and either PTBP1 or PTBP2. We also found that SUMO1 modification of PTBP2 was enhanced by Ubc9 (E2). The mutation of the sumoylation site (Lys137) of PTBP2 markedly inhibited its modification by SUMO1. Interestingly, in T98G glioma cells, the level of sumoylated PTBP2 was reduced compared to that of normal brain cells. Overall, this study shows that PTBP2 is posttranslationally modified by SUMO1. [BMB Reports 2014; 47(4): 233-238]

“Characterization Of A Novel Posttranslational Modification In Polypyrimidine Tract-binding Proteins By SUMO1.” Metadata:

  • Title: ➤  Characterization Of A Novel Posttranslational Modification In Polypyrimidine Tract-binding Proteins By SUMO1.
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  • Language: English

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4Posttranslational Modification Of Proteins : Expanding Nature's Inventory

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This article is from BMB Reports , volume 47 . Abstract Polypyrimidine tract-binding protein 1 (PTBP1) and its brainspecific homologue, PTBP2, are associated with pre-mRNAs and influence pre-mRNA processing, as well as mRNA metabolism and transport. They play important roles in neural differentiation and glioma development. In our study, we detected the expression of the two proteins in glioma cells and predicted that they may be sumoylated using SUMOplot analyses. We confirmed that PTBP1 and PTBP2 can be modified by SUMO1 with co-immunoprecipitation experiments using 293ET cells transiently co-expressing SUMO1 and either PTBP1 or PTBP2. We also found that SUMO1 modification of PTBP2 was enhanced by Ubc9 (E2). The mutation of the sumoylation site (Lys137) of PTBP2 markedly inhibited its modification by SUMO1. Interestingly, in T98G glioma cells, the level of sumoylated PTBP2 was reduced compared to that of normal brain cells. Overall, this study shows that PTBP2 is posttranslationally modified by SUMO1. [BMB Reports 2014; 47(4): 233-238]

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  • Title: ➤  Posttranslational Modification Of Proteins : Expanding Nature's Inventory
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1Posttranslational modification of proteins

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“Posttranslational modification of proteins” Metadata:

  • Title: ➤  Posttranslational modification of proteins
  • Author:
  • Language: English
  • Number of Pages: Median: 576
  • Publisher: Roberts and Co. Publishers
  • Publish Date:
  • Publish Location: Englewood, Colo

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  • First Year Published: 2005
  • Is Full Text Available: Yes
  • Is The Book Public: No
  • Access Status: Borrowable

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1Old-Time Makers of Medicine

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Dr. Walsh's <i>Old-Time Makers of Medicine</i> chronicles the history and development of modern medicine from ancient times up to the discovery of America. Throughout this historical guide, Dr. Walsh shows numerous examples of practices thought to be entirely modern that were clearly anticipated hundreds or thousands of years ago. Ancient healers sought to use the body's natural healing ability, rather than rely exclusively on external cures. Physicians even in ancient times relied on what is now recognized as the placebo effect.<br /><br /> Dr. Walsh also addresses training and certification in medicine. Medieval universities anticipate our modern medical textbooks with consolidated records of all research and independent investigations, to provide uniform training for students. Likewise, the reader will find that the ancients reacted to unsuccessful treatment in similar degrees to what might now be called medical malpractice suits.<br /><br /> The book is organized chronologically, beginning with the fall of the Roman Empire and growth of the early Christian Church. From there, Dr. Walsh details the development of medical knowledge and practice in Arabia, to Medieval and Renaissance Europe. The reader will also discover how modern cultures based much of their medical knowledge on ancient Greek teachings. The chapters on Arabian Physicians and Medieval Universities also discuss knowledge exchanged between Arabic and European cultures. Dr. Walsh exposes several misconceptions and misinterpretations of history, especially restrictions of medical research stemming from religious prohibitions. (Summary by LivelyHive)

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  • Number of Sections: 29
  • Total Time: 15:40:58

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2Despairing Lover

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LibriVox volunteers bring you 24 recordings of The Despairing Lover by William Walsh. This was the Fortnightly Poetry project for March 11, 2012.<br><br>William Walsh was an English poet and critic. It is not as a poet, however, but as the friend and correspondent of Alexander Pope that Walsh is remembered.<br>"Mr Walsh used to tell me," Pope says, "that there was one way left of excelling; for though we had several great poets, we never had any one great poet that was correct, and he desired me to make that my study and my aim." (Summary from Wikipedia )

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  • Language: English
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  • Format: Audio
  • Number of Sections: 24
  • Total Time: 0:33:37

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3Thirteenth: Greatest of Centuries

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It cannot but seem a paradox to say that the Thirteenth was the greatest of centuries. To most people the idea will appear at once so preposterous that they may not even care to consider it. A certain number, of course, will have their curiosity piqued by the thought that anyone should evolve so curious a notion. Either of these attitudes of mind will yield at once to a more properly receptive mood if it is recalled that the Thirteenth is the century of the Gothic cathedrals, of the foundation of the university, of the signing of Magna Charta, and of the origin of representative government with something like constitutional guarantees throughout the west of Europe. The cathedrals represent a development in the arts that has probably never been equaled either before or since. The university was a definite creation of these generations that has lived and maintained its usefulness practically in the same form in which it was then cast for the seven centuries ever since. The foundation stones of modern liberties are to be found in the documents which for the first time declared the rights of man during this precious period. (Summary from Preface)

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  • Title: ➤  Thirteenth: Greatest of Centuries
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  • Language: English
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  • Format: Audio
  • Number of Sections: 27
  • Total Time: 19:18:10

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  • Number of Sections: 27 sections

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4Bumper the White Rabbit

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All little boys and girls who love animals should become acquainted with Bumper the white rabbit. Bumper had many friends, such as Mr. Blind Rabbit, Fuzzy Wuzz and Goggle Eyes, his country cousins; and Bobby Gray Squirrel had his near cousins, Stripe the chipmunk and Webb the flying squirrel; while Buster and White Tail were favored with an endless number of friends and relatives. These books of Twilight Animal Stories are dedicated to all little boys and girls who love wild animals. So come out into the woods with me, and let us listen and watch, and I promise you it will be worth while. - Summary by Introduction of the book

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  • Title: Bumper the White Rabbit
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  • Language: English
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  • Format: Audio
  • Number of Sections: 16
  • Total Time: 02:22:00

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  • Number of Sections: 16 sections

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5Bumper the White Rabbit in the Woods

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In this second volume of the Twilight Animal series, we'll find out what happens to our friends Bumper, Fuzzy Wuzz, Goggle Eyes, and all the rest, after the events in the first book of the series, "Bumper the White Rabbit".

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  • Title: ➤  Bumper the White Rabbit in the Woods
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  • Language: English
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  • Format: Audio
  • Number of Sections: 15
  • Total Time: 02:11:20

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6Bumper the White Rabbit and His Friends

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In this fourth book of the Twilight Animal series, we find 16 stories of Bumper the white rabbit and the adventures he has with his friends in the forest.

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  • Title: ➤  Bumper the White Rabbit and His Friends
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  • Language: English
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  • Format: Audio
  • Number of Sections: 16
  • Total Time: 02:21:55

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  • Number of Sections: 16 sections

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7Bobby Gray Squirrel's Adventures

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In this sixth volume of the Twilight Animal series, we read about the adventures of Bobby the Gray Squirrel, who (together with his friends from the woods!) brings joy and happiness to a very special new friend. - Summary by Foon

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  • Title: ➤  Bobby Gray Squirrel's Adventures
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  • Language: English
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  • Format: Audio
  • Number of Sections: 16
  • Total Time: 02:27:06

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8Buster the Big Brown Bear

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In the seventh volume of the Twilight Animal series, we meet Buster the Bear, a cub who lives in a cave the woods with his mother. After an encounter with an unfriendly forest animal, he gets lost and captured, and has some marvelous adventures with humans. Will he make it back to the forest, or will he end up somewhere else? (Summary by Foon)

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  • Language: English
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  • Format: Audio
  • Number of Sections: 16
  • Total Time: 02:31:40

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9White Tail the Deer's Adventures

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White Tail is the son of Father Buck, who is the leader of the herd. He and his rival Young Black Buck get into all kinds of adventures, but they have to be wary of Puma the Mountain Lion and Timber Wolf! Will White Tail be able to keep away from them and follow in his father's hoofsteps? - Summary by Foon

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  • Title: ➤  White Tail the Deer's Adventures
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  • Language: English
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  • Format: Audio
  • Number of Sections: 16
  • Total Time: 02:27:25

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10Religion and Health

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In the introductory chapter and the one following we find the strong presentation of his thesis on the everlasting reality of religion. The chapter on Prayer, marked by absence of psychological speculation, treats practically of the naturalness and good sense of the constant habit of prayer, and of the value of prayer in all psychoneurosis....The chapter on the Bible and Health is of especial interest, with its argument that the sanitary laws of the Jews could have been no outcome of human development, but rather of Divine origin....The wide reading, extended experience, and specialized scholarship of the writer certify to the value of anything from his pen, and when we find a work of this kind as simple as a primer and as attractive as a story, we may well offer thanks for the boon. Nobody who values knowledge concerning the mysterious relation between holy living and bodily health should be without this book. - Summary by New Catholic World

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  • Title: Religion and Health
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  • Language: English
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  • Format: Audio
  • Number of Sections: 27
  • Total Time: 10:35:01

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11Story of Santa Klaus

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Who was Santa Klaus? Did he exist? In this study of custom and folklore, the author looks at the real and the legendary man, according to various traditions around the world. - Summary by LynneT

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  • Title: Story of Santa Klaus
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  • Language: English
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  • Format: Audio
  • Number of Sections: 17
  • Total Time: 03:22:17

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12Tea: Its History and Mystery

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Utility, not originality, has been aimed at in the compilation of this work. The obstacles and difficulties its author had met with in his endeavors to learn something of the article he was commissioned to sell when he first entered the Tea trade, the almost total lack of knowledge displayed by the average dealer in the commodity, allied to the numerous inquiries for a work containing “all about tea,” first prompted the undertaking. The material was collated at intervals, in a fragmentary manner, covering a period of over twenty years, and arranged amid the many interruptions incident to an active business life, subjected to constant revisions, repeated prunings and innumerable corrections, due mainly to the varying statements and conflicting opinions of admitted authorities in every branch of the subject. Still, as careful and judicious an arrangement of the data has been given as possible, a faithful effort being made to omit nothing that may prove useful, instructive or profitable to the expert, the dealer or general reader. Aware that many facts have been omitted, and many errors committed in its preparation, he still trusts that the pains he has taken to avoid both have not been in vain, that the former may be few, and the latter of no great importance. The work was compiled under impulse, not under inducement, a single line not being intended originally for the market, and is now being published solely for the benefit of those “whom it may concern.” Philadelphia, December, 1892. - Summary by From the Preface

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  • Title: Tea: Its History and Mystery
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  • Language: English
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  • Format: Audio
  • Number of Sections: 14
  • Total Time: 08:43:20

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  • Number of Sections: 14 sections

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13Makers of Modern Medicine

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The present volume is published at the solicitation of many friends who have read the articles contained in it as they appeared at various times in magazines and who deemed that they were worth preservation in a more permanent form. The only possible claim for its filling a want lies in the fact that it presents these workers in medicine not only as scientists but also and especially as men, in relation to their environment, social, religious and educational. - Summary by Preface

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  • Number of Sections: 29

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