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This article is from Nucleic Acids Research , volume 40 . Abstract Most restriction endonucleases, including FokI, interact with two copies of their recognition sequence before cutting DNA. On DNA with two sites they act in cis looping out the intervening DNA. While many restriction enzymes operate symmetrically at palindromic sites, FokI acts asymmetrically at a non-palindromic site. The directionality of its sequence means that two FokI sites can be bridged in either parallel or anti-parallel alignments. Here we show by biochemical and single-molecule biophysical methods that FokI aligns two recognition sites on separate DNA molecules in parallel and that the parallel arrangement holds for sites in the same DNA regardless of whether they are in inverted or repeated orientations. The parallel arrangement dictates the topology of the loop trapped between sites in cis: the loop from inverted sites has a simple 180° bend, while that with repeated sites has a convoluted 360° turn. The ability of FokI to act at asymmetric sites thus enabled us to identify the synapse geometry for sites in trans and in cis, which in turn revealed the relationship between synapse geometry and loop topology.

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This week’s your last chance to catch the re-loop of The Sound of Grand Theft Auto, our radio takeover with @RockstarGames looking into the musical history of their flagship series. Re-looping via Infinite Mixtapes ➝ https://t.co/8K7pSoA4JU https://t.co/2RHzPYTgTL Source: https://twitter.com/NTSlive/status/1354142059431677953 Uploader: NTS Radio

Looping, or multi-year teaching, is the pedagogical practice of allowing students and teachers to remain together for 2 or more years. This 2-year case study investigated looping practices and perceptions of looping among eighth-grade middle school students, teachers, and parents. Data from two middle schools were collected in the second year of looping through interviews with 12 students, 4 teachers, and 11 parents; surveys of 214 students, 9 teachers, and 75 parents; and videotapes of looping classrooms. Findings revealed that 80 percent of participants reported positive results from looping related to ease of transition into eighth grade, enhanced sense of knowing about the students' needs, better accountability, more sustained peer and teacher-student relationships, more effective curriculum planning, and enhanced parent involvement. Twenty percent of students and parents preferred having different teachers every year. Students believed they should have a choice about looping. (Ten appendices include interview and survey questions and a compilation of student survey responses. Contains 183 references.) (Author/KB)

Looping the Loop (German: Die Todesschleife) is a 1928 German silent thriller film directed by Arthur Robison and starring Werner Krauss, Jenny Jugo and Warwick Ward.[1] The film was produced by UFA. It was shot at the Babelsberg Studios in Berlin and on location in London. The film's sets were designed by the art directors Robert Herlth and Walter Röhrig. As with UFA's Variety, Paramount Pictures handled the film's American distribution as part of the Parufamet agreement. Paramount prepared a sound version for distribution in English speaking countries. While the sound version has no audible dialog, it featured a synchronized musical score with sound effects using both the sound-on-disc and sound-on-film process. from wikipedia