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  • Title: ➤  Stability Indicating Method Development And Validation Of Deferiprone In Pharmaceutical Dosage Form By RP-HPLC
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  • Internet Archive ID: jipbsv-4-i-209-n

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<div>The objective of this present is to develop a simple, precise, accurate stability indicating method </div><div>for the estimation of deferiprone in formulation by using RP-HPLC. The separation was </div><div>achieved on Inertsil ODS C18, 250x 4.6mm, 5µm i.d. column using 60 volumes of Mixed </div><div>Phosphate  buffer(KH2PO4+K2HPO4) pH 3.0 and 40 volumes of  methanol as mobile phase and </div><div>at a flow rate of 1.0  mL/min. Detection was carried out using a PDA detector at 280nm. The </div><div>method was validated for accuracy, precision, specificity, linearity and sensitivity. The total </div><div>chromatographic analysis time per sample was about 5 min with deferiprone eluting at retention </div><div>time of about 4.980 min. The method was validated as per ICH guide lines. Stability studies </div><div>reported absence of impurities at the peak retention time. The drug was stable to different </div><div>conditions like acidic, alkali, thermal, oxidative and photolytic conditions. Validation studies </div><div>demonstrated that the proposed HPLC method is simple, specific, rapid, reliable and </div><div>reproducible. The standard curves were linear over the concentration range of 75-125µg/mL. </div><div>The LOD and LOQ values for deferiprone were 3.91 and 11.8 µg/mL, respectively. The </div><div>percentage recovery was found to be 97.3 to 98.0 and the %RSD for precision was found to be </div><div>0.5. The high recovery and low relative standard deviation confirm the suitability of the </div><div>proposed  method for the determination  of  deferiprone in  bulk  and  capsule  dosage  forms</div>

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