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methods and protocols

"Shotgun proteomics" was published by Humana Press in 2014 - New York, it has 492 pages and the language of the book is English.


“Shotgun proteomics” Metadata:

  • Title: Shotgun proteomics
  • Author:
  • Language: English
  • Number of Pages: 492
  • Publisher: Humana Press
  • Publish Date:
  • Publish Location: New York

“Shotgun proteomics” Subjects and Themes:

Edition Specifications:

  • Pagination: xiv, 492 pages

Edition Identifiers:

AI-generated Review of “Shotgun proteomics”:


"Shotgun proteomics" Table Of Contents:

  • 1- Survey of shotgun proteomics -- Fabio Cesar Sousa Nogueira and Gilberto B. Domont
  • 2- LC-MALDI-TOF/TOF for shotgun proteomics -- Patricia Fernández-Puente ... [et al.]
  • 3- Fully automatable multidimensional reversed-phase liquid chromatography with online tandem mass spectrometry -- Maggie P.Y. Lam ... [et al.]
  • 4- GeLC-MS/MS analysis of complex protein mixtures -- Monika Dzieciatkowska, Ryan Hill, and Kirk C. Hansen
  • 5- IPG strip-based peptide fractionation for shotgun proteomics -- Murat Eravci, Christian Sommer, and Matthias Selbach
  • 6- SILAC yeast : from labeling to comprehensive Proteome Quantification -- Lyris M. F. de Godoy
  • 7- Analysis of proteome dynamics in mice by isotopic labeling -- John C. Price and Sina Ghaemmaghami
  • 8- Stable isotope labeling in mammals (SILAM) -- Daniel B. McClatchy and John R. Yates III
  • 9- Analysis of individual protein turnover in live animals on a proteome-wide scale -- Stefan Reckow and Christian Webhofer
  • 10- Determining protein subcellular localization in mammalian cell culture with biochemical fractionation and iTRAQ 8-plex quantification -- Andy Christoforou, Alfonso Martinez Arias, and Kathryn S. Lilley
  • 11- Brain quantitative proteomics combining GeLC-MS and isotope-coded protein labeling (ICPL) -- Giuseppina Maccarrone, Maria Lebar, and Daniel Martins-de-Souza
  • 12- Employing TMT quantification in a shotgun-MS platform -- Darragh P. O'Brien and John F. Timms
  • 13- Employing TMT quantification in shotgun-MS proteomic analysis : a focus on skeletal muscle -- Bruno Menezes de Oliveira
  • 14- Spectral counting label-free proteomics -- Liisa Arike and Lauri Peil
  • 15- Quantification of proteins by label-free LC-MSE -- Alon Savidor and Yishai Levin
  • 16- Bioinformatics for proteomics : opportunities at the interface between the scientists, their experiments, and the community -- Marc Vaudel ... [et al.]
  • 17- Identification of DNA damage checkpoint-dependent protein interactions in Saccharomyces cerevisiae using quantitative mass spectrometry -- Francisco M. Bastos de Oliveira and Marcus B. Smolka
  • 18- Application of shotgun proteomics for discovery-driven protein-protein interaction -- Livia Goto-Silva ... [et al.]
  • 19- Mapping protein complexes using covalently linked antibodies and isobaric mass tags -- Antje Dittmann ... [et al.]
  • 20- Biomarker verification using selected reaction monitoring and shotgun proteomics -- Angel Mauricio Castro-Gamero, Clarice Izumi, and José César Rosa
  • 21- Use of universal stable isotope labeling by amino acids in cell culture (SILAC)-based selected reaction monitoring (SRM) approach for verification of breast cancer-related protein markers -- Ning Qing Liu ... [et al.]
  • 22- Secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM) -- Vitor M. Faça ... [et al.]
  • 23- Preparation of heteroelement-incorporated and stable isotope-labeled protein standards for quantitative proteomics -- Anna Konopka ... [et al.]
  • 24- One-source peptide/phosphopeptide ratio standards for accurate and site-specific determination of the degree of phosphorylation -- Martin E. Boehm, Bettina Hahn, and Wolf D. Lehmann
  • 25- Quantitative glycoproteomics for N-glycoproteome profiling -- Sheng Pan
  • 26- Practical recipe to survey phosphoproteomes -- William C. Edelman ... [et al.]
  • 27- Quantitation of the phosphoproteome using the library-assisted eXtracted ion chromatogram (LAXIC) strategy -- Justine V. Arrington, Liang Xue, and W. Andy Tao
  • 28- Fast, efficient, and quality-controlled phosphopeptide enrichment from minute sample amounts using titanium dioxide -- Clarissa Dickhut, Sonja Radau, and René P. Zahedi
  • 29- Quantifying small molecule-induced changes in cellular protein expression and posttranslational modifications using isobaric mass tags -- Isabelle Becher, Maria Fälth Savitski, and Marcus Bantscheff
  • 30- Analysis of protein structure by cross-linking combined with mass spectrometry -- Evgeniy V. Petrotchenko ... [et al.]
  • 31- Top-down proteomics by means of orbitrap mass spectrometry -- Kai Scheffler.

"Shotgun proteomics" Description:

The Open Library:

Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified, and characterized in a high throughput manner. Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics, and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species.

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