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"Quantitative Determination Of L-DOPA In Seeds Of Mucuna Pruriens Germplasm By High Performance Thin Layer Chromatography." and the language of the book is English.


“Quantitative Determination Of L-DOPA In Seeds Of Mucuna Pruriens Germplasm By High Performance Thin Layer Chromatography.” Metadata:

  • Title: ➤  Quantitative Determination Of L-DOPA In Seeds Of Mucuna Pruriens Germplasm By High Performance Thin Layer Chromatography.
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  • Language: English

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  • Internet Archive ID: pubmed-PMC3374567

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This article is from <a href="//archive.org/search.php?query=journaltitle%3A%28Indian%20Journal%20of%20Pharmaceutical%20Sciences%29" rel="ugc nofollow">Indian Journal of Pharmaceutical Sciences</a>, <a href="//archive.org/search.php?query=journaltitle%3A%28Indian%20Journal%20of%20Pharmaceutical%20Sciences%29%20AND%20volume%3A%2873%29" rel="ugc nofollow">volume 73</a>.<h2>Abstract</h2>Mucuna pruriens Linn. is an important medicinal plant used for treatment of Parkinson's disease and many others in ancient Indian medical system. L-DOPA extracted from seeds of Mucuna is a constituent of more than 200 indigenous drug formulations and is more effective as drug than the synthetic counterpart. A densitometric high performance thin-layer chromatographic (HPTLC) method was developed for quantification of L-DOPA content present in the seeds extract. The method involves separation of L-DOPA on precoated silica gel 60 GF254 HPTLC plates using a solvent system of n-butanol-acetic-acid-water (4:1:1, v/v) as the mobile phase. Quantification was done at 280 nm using absorbance reflectance mode. Linearity was found in the concentration range of 100 to 1000 ng/spot with the correlation coefficient value of 0.9980. The method was validated for accuracy, precision and repeatability. Mean recovery was 100.89%. The LOD and LOQ for L-DOPA determination were found to be 3.41 ng/spot and 10.35 ng/spot respectively. The proposed HPTLC method was found to be precise, specific and accurate for quantitative determination of L-DOPA. It can be used for rapid screening of large germplasm collections of Mucuna pruriens for L-DOPA content. The method was used to study variation in fifteen accessions of Mucuna germplasm collected from different geographical regions.

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