"OligArch: A Software Tool To Allow Artificially Expanded Genetic Information Systems (AEGIS) To Guide The Autonomous Self-assembly Of Long DNA Constructs From Multiple DNA Single Strands." - Information and Links:

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"OligArch: A Software Tool To Allow Artificially Expanded Genetic Information Systems (AEGIS) To Guide The Autonomous Self-assembly Of Long DNA Constructs From Multiple DNA Single Strands." and the language of the book is English.


“OligArch: A Software Tool To Allow Artificially Expanded Genetic Information Systems (AEGIS) To Guide The Autonomous Self-assembly Of Long DNA Constructs From Multiple DNA Single Strands.” Metadata:

  • Title: ➤  OligArch: A Software Tool To Allow Artificially Expanded Genetic Information Systems (AEGIS) To Guide The Autonomous Self-assembly Of Long DNA Constructs From Multiple DNA Single Strands.
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  • Language: English

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  • Internet Archive ID: pubmed-PMC4142867

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This article is from <a href="//archive.org/search.php?query=journaltitle%3A%28Beilstein%20Journal%20of%20Organic%20Chemistry%29" rel="nofollow">Beilstein Journal of Organic Chemistry</a>, <a href="//archive.org/search.php?query=journaltitle%3A%28Beilstein%20Journal%20of%20Organic%20Chemistry%29%20AND%20volume%3A%2810%29" rel="nofollow">volume 10</a>.<h2>Abstract</h2>Synthetic biologists wishing to self-assemble large DNA (L-DNA) constructs from small DNA fragments made by automated synthesis need fragments that hybridize predictably. Such predictability is difficult to obtain with nucleotides built from just the four standard nucleotides. Natural DNA's peculiar combination of strong and weak G:C and A:T pairs, the context-dependence of the strengths of those pairs, unimolecular strand folding that competes with desired interstrand hybridization, and non-Watson–Crick interactions available to standard DNA, all contribute to this unpredictability. In principle, adding extra nucleotides to the genetic alphabet can improve the predictability and reliability of autonomous DNA self-assembly, simply by increasing the information density of oligonucleotide sequences. These extra nucleotides are now available as parts of artificially expanded genetic information systems (AEGIS), and tools are now available to generate entirely standard DNA from AEGIS DNA during PCR amplification. Here, we describe the OligArch (for "oligonucleotide architecting") software, an application that permits synthetic biologists to engineer optimally self-assembling DNA constructs from both six- and eight-letter AEGIS alphabets. This software has been used to design oligonucleotides that self-assemble to form complete genes from 20 or more single-stranded synthetic oligonucleotides. OligArch is therefore a key element of a scalable and integrated infrastructure for the rapid and designed engineering of biology.

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