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“Measurement Of Carbonic Anhydrase I And II Isozymes In Feces As A Marker Of Occult Blood In Horses With Intestinal Tract Bleeding.” Metadata:

  • Title: ➤  Measurement Of Carbonic Anhydrase I And II Isozymes In Feces As A Marker Of Occult Blood In Horses With Intestinal Tract Bleeding.
  • Authors: ➤  
  • Language: English

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  • Internet Archive ID: pubmed-PMC4013991

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"Measurement Of Carbonic Anhydrase I And II Isozymes In Feces As A Marker Of Occult Blood In Horses With Intestinal Tract Bleeding." Description:

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This article is from <a href="//archive.org/search.php?query=journaltitle%3A%28Journal%20of%20equine%20science%29" rel="ugc nofollow">Journal of equine science</a>, <a href="//archive.org/search.php?query=journaltitle%3A%28Journal%20of%20equine%20science%29%20AND%20volume%3A%2824%29" rel="ugc nofollow">volume 24</a>.<h2>Abstract</h2>Although endoscopy is the definitive diagnostic method for the detection of colonic ulcers, the equipment required for performing the test is costly and difficult to use. Therefore, a simple cost-effective and reliable screening test for intestinal tract bleeding is needed. To this end, we measured carbonic anhydrase isozymes (CA-I and CA-II) originating from erythrocytes by ELISA in order to determine if they could be used as markers of occult blood in feces. For fecal extract preparation, 2 g of feces were mixed with 4 ml of 0.01 M Tris-HCl (pH 8.0) containing 0.01% thimerosal. The concentrations of CA-I and CA-II in the fecal samples of 13 clinically normal racehorses were found to be 30.0 ± 10.0 and 34.0 ± 13.0 ng/ml, respectively. Increased concentrations of CA-I were detected in the fecal samples of 5 horses after blood administration; however, no increase was observed in CA-II. The concentrations of CA-I and CA-II in the fecal samples of 88 racehorses with clinical signs of equine gastric ulcer syndrome (EGUS) were 115.3 ± 79.0 and 41.0 ± 42.0 ng/ml, respectively. Thus, our results indicate that CA isozymes can be useful as markers of occult blood in the fecal samples of horses with intestinal tract bleeding.

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