Matrix metalloproteinase protocols - Info and Reading Options

"Matrix metalloproteinase protocols" Table Of Contents:

• 1- Metalloproteases and the degradome / Alejandro P. Ugalde ... [et al.]
• 2- Mouse models of MMP and TIMP function / Sean E. Gill ... [et al.]
• 3- Expression of recombinant MMP-28 in mammalian cells / Ursula R. Rodgers and Ian M. Clark
• 4- Expression of recombinant matrix metalloproteinases in Escherichia coli / L. Jack Windsor and Darin L. Steele
• 5- Expression of recombinant ADAMTS in insect cells / Gavin C. Jones, Mireille N. Vankemmelbeke and David J. Buttle
• 6- Expression and purification of membrane-type MMPs / Jing Nie and Duanqing Pei
• 7- Refolding of TIMP-2 from Escherichia coli inclusion bodies / Richard A. Williamson
• 8- Purification of MMPs and TIMPs / Ken-ichi Shimokawa and Hideaki Nagase
• 9- Real-time PCR expression profiling of MMPs and TIMPs / Caroline J. Pennington and Dylan R. Edwards
• 10- Analysis of the degradome with the CLIP-CHIP microarray / Reinhild Kappelhoff, Ulrich auf dem Keller, and Christopher M. Overall
• 11- In situ hybridization for metalloproteinases and their inhibitors / Tiina L. Hurskainen and Suneel S. Apte
• 12- Immunohistochemistry of MMPs and TIMPs / Yasunori Okada
• 13- Single nucleotide polymorphism genotyping in MMP genes : the 5' nuclease assay / Ross Laxton and Shu Ye
• 14- Methods for studying activation of matrix metalloproteinases / Vera Knäuper and Gillian Murphy
• 15- Assay of matrix metalloproteinases against matrix substrates / Tim E. Cawston, Rachel L. Lakey, and Andrew D. Rowan
• 16- Zymography and reverse zymography for detecting MMPs and TIMPs / Susan P. Hawkes, Hongxia Li, and Gary T. Taniguchi, with contributions from Marc A. Lafleur
• 17- In situ zymography / Sarah J. George and Jason L. Johnson
• 18- Near-infrared optical proteolytic beacons for in vivo imaging of matrix metalloproteinase activity / J. Oliver McIntyre, Randy L. Scherer, and Lynn M. Matrisian
• 19- Neoepitope antibodies against MMP-cleaved and aggrecanase-cleaved aggrecan / Amanda J. Fosang ... [et al.]
• 20- In vitro model of cartilage degradation / Wang Hui and Tim E. Cawston
• 21- Immunoassays for collagenase-mediated cleavage of type I and II collagens / R. Clark Billinghurst, Mirela Ionescu, and A. Robin Poole
• 22- Collagen degradation assays / Anthony P. Hollander
• 23- Analysis of MMP-dependent cell migration and invasion / Ralf Palmisano and Yoshifumi Itoh
• 24- Using fluorogenic peptide substrates to assay matrix metalloproteinases / Gregg B. Fields
• 25- Kinetic analysis of the inhibition of matrix metalloproteinases : lessons from the study of tissue inhibitors of metalloproteinases / Frances Willenbrock, Daniel A. Thomas, and Augustin Amour
• 26- Identification of cellular MMP substrates using quantitative proteomics : isotope-coded affinity tags (ICAT) and isobaric tags for relative and absolute quantification (iTRAQ) / Georgina S. Butler ... [et al.]
• 27- Mechanism-based profiling of MMPs / Jed F. Fisher and Shahriar Mobashery.

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