"GFP whole cell microbial biosensors" - Information and Links:

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scale-up and scale-down effects on biopharmaceutical processes

"GFP whole cell microbial biosensors" was published by ASME Press in 2013 - New York, N.Y, it has 43 pages and the language of the book is English.


“GFP whole cell microbial biosensors” Metadata:

  • Title: ➤  GFP whole cell microbial biosensors
  • Author:
  • Language: English
  • Number of Pages: 43
  • Publisher: ASME Press
  • Publish Date:
  • Publish Location: New York, N.Y

“GFP whole cell microbial biosensors” Subjects and Themes:

Edition Specifications:

  • Pagination: 43 p.

Edition Identifiers:

AI-generated Review of “GFP whole cell microbial biosensors”:


"GFP whole cell microbial biosensors" Table Of Contents:

  • 1- Interaction between fluid flow and microbial cells : importance of the operating scale
  • 2- Stochastic simulation of the displacement of microbial cells along concentration field
  • 3- Experimental results gained from the physiological response of GFP biosensors in scale-down conditions
  • 4- Another source of information : protein leakage and the study of the secretome.

"GFP whole cell microbial biosensors" Description:

The Open Library:

Two strategies are usually considered for the optimization of microbial bioprocesses. The first one involves genetic or metabolic engineering of the target microbial strains in order to improve its production efficiency or its tolerance to adverse conditions. The second one is based on the chemical engineering improvement of the bioreactors and scaling-up rules. This work is more particularly dedicated to this second class of parameters. Recent developments in bioreactor technologies follow the scaling-out principle, i.e. carrying out several cultures in parallel with controlled conditions for screening purposes. Several mini-bioreactor concepts, i.e. bioreactor with working volume of 1 to 100 mL with controlling devices, have been developed following this principle. In general, chemical engineering similarities between conventional stirred bioreactors and their miniature equivalent are well characterized. However, the actual scaling-up rules are not able to cope with the complexity of the microbial stress response. Indeed, microbial stress response still remains not completely understood considering the process perturbations and the environmental fluctuations accompanying the scaling-up to industrial bioreactors. At this time, this kind of response can only be experimentally predicted by using scale-down bioreactors, i.e. lab-scale bioreactors designed in order to reproduce mixing imperfections that have to be expected at large-scale. However, the use of such an approach is time consuming and requires an experimented staff to elaborate the scaling-down protocols. Indeed, bioprocess development involves several steps which cannot be necessarily linked with each other considering the different cultivation equipment used--

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