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This article is from Nucleic Acids Research , volume 41 . Abstract Bacterial RNase J and eukaryal cleavage and polyadenylation specificity factor (CPSF-73) are members of the β-CASP family of ribonucleases involved in mRNA processing and degradation. Here we report an in-depth phylogenomic analysis that delineates aRNase J and archaeal CPSF (aCPSF) as distinct orthologous groups and establishes their repartition in 110 archaeal genomes. The aCPSF1 subgroup, which has been inherited vertically and is strictly conserved, is characterized by an N-terminal extension with two K homology (KH) domains and a C-terminal motif involved in dimerization of the holoenzyme. Pab-aCPSF1 (Pyrococcus abyssi homolog) has an endoribonucleolytic activity that preferentially cleaves at single-stranded CA dinucleotides and a 5′–3′ exoribonucleolytic activity that acts on 5′ monophosphate substrates. These activities are the same as described for the eukaryotic cleavage and polyadenylation factor, CPSF-73, when engaged in the CPSF complex. The N-terminal KH domains are important for endoribonucleolytic cleavage at certain specific sites and the formation of stable high molecular weight ribonucleoprotein complexes. Dimerization of Pab-aCPSF is important for exoribonucleolytic activity and RNA binding. Altogether, our results suggest that aCPSF1 performs an essential function and that an enzyme with similar activities was present in the last common ancestor of Archaea and Eukarya.

This article is from Nucleic Acids Research , volume 41 . Abstract Bacterial RNase J and eukaryal cleavage and polyadenylation specificity factor (CPSF-73) are members of the β-CASP family of ribonucleases involved in mRNA processing and degradation. Here we report an in-depth phylogenomic analysis that delineates aRNase J and archaeal CPSF (aCPSF) as distinct orthologous groups and establishes their repartition in 110 archaeal genomes. The aCPSF1 subgroup, which has been inherited vertically and is strictly conserved, is characterized by an N-terminal extension with two K homology (KH) domains and a C-terminal motif involved in dimerization of the holoenzyme. Pab-aCPSF1 (Pyrococcus abyssi homolog) has an endoribonucleolytic activity that preferentially cleaves at single-stranded CA dinucleotides and a 5′–3′ exoribonucleolytic activity that acts on 5′ monophosphate substrates. These activities are the same as described for the eukaryotic cleavage and polyadenylation factor, CPSF-73, when engaged in the CPSF complex. The N-terminal KH domains are important for endoribonucleolytic cleavage at certain specific sites and the formation of stable high molecular weight ribonucleoprotein complexes. Dimerization of Pab-aCPSF is important for exoribonucleolytic activity and RNA binding. Altogether, our results suggest that aCPSF1 performs an essential function and that an enzyme with similar activities was present in the last common ancestor of Archaea and Eukarya.

This article is from Nucleic Acids Research , volume 41 . Abstract Bacterial RNase J and eukaryal cleavage and polyadenylation specificity factor (CPSF-73) are members of the β-CASP family of ribonucleases involved in mRNA processing and degradation. Here we report an in-depth phylogenomic analysis that delineates aRNase J and archaeal CPSF (aCPSF) as distinct orthologous groups and establishes their repartition in 110 archaeal genomes. The aCPSF1 subgroup, which has been inherited vertically and is strictly conserved, is characterized by an N-terminal extension with two K homology (KH) domains and a C-terminal motif involved in dimerization of the holoenzyme. Pab-aCPSF1 (Pyrococcus abyssi homolog) has an endoribonucleolytic activity that preferentially cleaves at single-stranded CA dinucleotides and a 5′–3′ exoribonucleolytic activity that acts on 5′ monophosphate substrates. These activities are the same as described for the eukaryotic cleavage and polyadenylation factor, CPSF-73, when engaged in the CPSF complex. The N-terminal KH domains are important for endoribonucleolytic cleavage at certain specific sites and the formation of stable high molecular weight ribonucleoprotein complexes. Dimerization of Pab-aCPSF is important for exoribonucleolytic activity and RNA binding. Altogether, our results suggest that aCPSF1 performs an essential function and that an enzyme with similar activities was present in the last common ancestor of Archaea and Eukarya.

1House of a Thousand Candles

By Meredith Nicholson

A top ten bestseller of 1906, <i>The House of a Thousand Candles</i> is part adventure/mystery and part romance. The book begins with young Jack Glenarm returning from various exploits in Europe and Africa for the reading of his grandfather’s will. In it, he stands to inherit his grandfather’s estate, but only if he can remain for one year in residence at the old man’s unfinished “House of a Thousand Candles” in Annandale, Indiana, with only his grandfather’s mysterious valet for company. If he violates the terms of the will, the house will go to a young woman, heretofore unknown to him, whom the will also forbids Jack to marry if he wants to retain his inheritance. This all sounds very mundane to Jack and he fully expects to be quite bored in very short order. Soon after Jack’s arrival at Glenarm House, however, various strange occurrences ensue, and he soon finds himself absorbed in the most lively adventure of his life! (summary by J. M. Smallheer)

2House of a Thousand Candles (version 2)

By Meredith Nicholson

A reputedly wealthy and eccentric old man dies in Vermont. His home, the House of a Thousand Candles, so called for the owner's preference to candle light, is left empty save a faithful servant -- his fortune mysteriously vanished, though rumored to still have been hidden in the house somewhere. John Glenarm, the late old man's grandson, stands to inherit the estate (and so the secret fortune) under the stipulation that he live in the house for one year. If he fails, the house will be forfeited and awarded to Marian Devereaux, the niece of the nun who operates the nearby Saint Agatha's School for girls. Mister Pickering, the executor of the estate and childhood rival of John's, decides to find the hidden treasure before young Glenarm does.

3Port of Missing Men

By Meredith Nicholson

Shirley Claiborne is fascinated by the tall handsome man named John Armitage who seemed to follow her and her brother, Captain Claiborne, as they traveled around Europe. Count von Stroebel had urged Armitage to do something for Austria. Now von Stroebel was dead – cut down by an assassin’s bullet – and Jules Chauvenet is one step closer to seeing the corrupt and worthless Francis ascend to the throne. When Shirley and Captain Claiborne sail for their home in Washington D.C., Armitage follows them. Monsieur Chauvenet also follows, but is he following Shirley or the mysterious John Armitage? And just who is John Armitage? (Introduction by MaryAnn)

The object of the present venture is to do something to revive the interest of the ordinary English reader in Ariosto. The present volume is intended to give some of the chief stories of the "Orlando Furioso" in such a way as to bring out also the main plot. The "Orlando Furioso" is a conglomeration of stories of all kinds, from the most delicate and ideal romance to the broadest humor. (Summary taken from the author's introduction)

5Broken Barriers

By Meredith Nicholson

Life abruptly changes for young socialite, Grace Durland., when her father goes bankrupt and she is forced to earn a living. Thrust into a new life, she meets -- and falls in love with -- a married man. She faces condemnation from friends and family alike, and although Ward Trenton reciprocates her feelings, his wife refuses him a divorce. Once again, fate intervenes in the form of a serious accident. - Summary by Lynne Thompson

6Hope of Happiness

By Meredith Nicholson

A hot and sultry day in the mid-west finds Bruce Storrs trying to cool off and clear his head. He has received a troubling death-bed letter from his mother and needs to decide if it is true, or the ramblings of a sick mind. If it is, indeed, true, as he suspects, what is he to do about it? And what will be his reception? - Summary by Lynne T

7Mystics of Islam

By Reynold A. Nicholson

Exploring Islamic mysticism, specifically Sufism, this book aims to provide an introduction to this difficult subject. Delving into the mystics' experiences and philosophies from the eighth century on. It reveals the how the concepts of divine love, knowledge, and the individual's spiritual journey toward God within Islamic thought. (Summary by Jessie Percival)

8Little Brown Jug at Kildare

By Meredith Nicholson

Two men in search of adventure and romance set off on their own journeys. Only to find themselves been drawn together to solve issues arising in the States of North and South Carolina when both of their Governors go missing.