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Rapid%2c Cost Effective%2c Sensitive And Quantitative Detection Of Acinetobacter Baumannii From Pneumonia Patients. by Nomanpour%2c B

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1Rapid, Cost-effective, Sensitive And Quantitative Detection Of Acinetobacter Baumannii From Pneumonia Patients.

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This article is from Iranian Journal of Microbiology , volume 3 . Abstract Background and Objectives: Pneumonia with Acinetobacter baumannii has a major therapeutic problem in health care settings. Decision to initiate correct antibiotic therapy requires rapid identification and quantification of organism. The aim of this study was to develop a rapid and sensitive method for direct detection of A. baumannii from respiratory specimens. Materials and Methods: A Taqman real time PCR based on the sequence of blaoxa-51 was designed and used for direct detection of A. baumannii from 361 respiratory specimens of patients with pneumonia. All specimens were checked by conventional bacteriology in parallel. Results: The new real time PCR could detect less than 200 cfu per ml of bacteria in specimens. There was agreement between the results of real time PCR and culture (Kappa value 1.0, p value

“Rapid, Cost-effective, Sensitive And Quantitative Detection Of Acinetobacter Baumannii From Pneumonia Patients.” Metadata:

  • Title: ➤  Rapid, Cost-effective, Sensitive And Quantitative Detection Of Acinetobacter Baumannii From Pneumonia Patients.
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  • Language: English

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