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In the Membrane Bio-Reactor waste-water treatment system,The water production of membrane modules is an important parameter to measure the working efficiency of MBR system, while the internal pressure series MBR is a common membrane module connection method. Therefore, the calculation and analysis of the water production of internal pressure series MBR system by computational fluid dynamics is carried out. In the analysis process, the geometric model of the internal pressure series MBR system is first established by the CFD preprocessor and the mesh is divided. Then the CFD solver is used to calculate the water production of the model. Finally, the CFD post processor is used to present the calculation results in an image. On this basis, this paper has carried out a large number of experimental analysis. In the analysis process, this paper compares the calculation results of the solver with the actual operating system results of MBR. The comparison results show that the calculation results of the solver are basically consistent with the actual water production of membrane modules, and have higher calculation accuracy. It achieves the purpose of accurately calculating the water production of membrane modules, and solves the problem of measuring the working efficiency of MBR system by calculating the water production of membrane modules. which has certain reference value for MBR engineering design and research.