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Two-dimensional, incompressible, spatially developing mixing layer simulations are performed at Re = 10 2 and 10 4 with two classes of perturbations applied at the inlet boundary; (1) combinations of discrete modes from linear stability theory, and (2) a broad spectrum of modes derived from experimentally measured velocity spectra. The effect of the type and strength of inlet perturbations on vortex dynamics and time-averaged properties are explored. Two-point spatial velocity and autocorrelations are used to estimate the size and lifetime of the resulting coherent structures and to explore possible feedback effects. The computed time-averaged properties such as mean velocity profiles, turbulent statistics, and spread rates show good agreement with experimentally measured values. It is shown that by forcing with a broad spectrum of modes derived from an experimental energy spectrum many experimentally observed phenomena can be reproduced by a 2-D simulation. The strength of the forcing merely affected the length required for the dominant coherent structures to become fully-developed. Thus intensities comparable to those of the background turbulence in many wind tunnel experiments produced the same results, given sufficient simulation length. Mixing layers, Numerical simulation, Spatial simulation.