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We have examined the immunohistochemical localization of over 12 different neurotransmitters in all of the nervous system components of two different marine molluscs, Aplysia and Pleurobranchaea. To do this for each neurotransmitter, we made complete serial histological sections of all major ganglia in each nervous system. Localizations were performed using immunohistochemistry and fluorescence microscopy. Since many of the commercially available antisera proved to be nonspecific, we generated 11 antisera in our laboratory. One of the major achievements occurred in our work on acetylcholine, which our previous AFOSR-funded work implicated as having an important role in associative learning in Pleurobranchaea through muscarinic receptors.