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The purpose of the work was to determine the validity limits of a previously derived theoretical model of an electrostatic precipitator air sampler. The theoretical model was investigated by insertion of radioactive micronsize microspheres into a closed-loop wind tunnel, whose airstream flows into an electrostatic precipitator. The precipitation pattern on the collection plates was determined by the pattern of radioactivity. Ideally, the particles should be segregated by size. The results were compared to the theoretical predictions which are calculated with a digital computer program. Conclusions are that (1) the results are reproducible within experimental error; (2) the physical phenomena in the precipitator can be described by mathematical models as suggested in the original theoretical study; (3) mathematical models being used are probably imperfect; and (4) investigation of the validity of the theoretical model should continue.