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This article is from PLoS ONE , volume 9 . Abstract Background: Intrauterine growth restriction (IUGR) is thought to lead to fetal programming that in turn contributes to developmental changes of many organs postnatally. There is evidence that IUGR is a risk factor for the development of metabolic and cardiovascular disease later in life. A higher incidence of breast cancer was also observed after IUGR. This could be due to changes in mammary gland developmental pathways. We sought to characterise IUGR-induced alterations of the complex pathways of mammary development at the level of the transcriptome in a rat model of IUGR, using pathways analysis bioinformatics. Methodology/Principal Findings: We analysed the mammary glands of Wistar rats with IUGR induced by maternal low protein (LP) diet at the beginning (d21) and the end (d28) of pubertal ductal morphogenesis. Mammary glands of the LP group were smaller in size at d28, however did not show morphologic changes. We identified multiple differentially expressed genes in the mammary gland using Agilent SurePrint arrays at d21 and d28. In silico analysis was carried out using Ingenuity Pathways Analysis. In mammary gland tissue of LP rats at d21 of life a prominent upregulation of WT1 and CDKN1A (p21) expression was observed. Differentially regulated genes were associated with the extracellular regulated kinase (ERK)-1/-2 pathway. Western Blot analysis showed reduced levels of phosphorylated ERK-1/-2 in the mammary glands of the LP group at d21. To identify possible changes in circulating steroid levels, serum LC-Tandem mass-spectrometry was performed. LP rats showed higher serum progesterone levels and an increased corticosterone/dehydrocorticosterone-ratio at d28. Conclusions/Significance: Our data obtained from gene array analysis support the hypothesis that IUGR influences pubertal development of the rat mammary gland. We identified prominent differential regulation of genes and pathways for factors regulating cell cycle and growth. Moreover, we detected new pathways which appear to be programmed by IUGR.

1Domestic Cookery, Useful Receipts, and Hints to Young Housekeepers

By Elizabeth E. Lea

The compiler of [this book] having entered early in life upon a train of duties, was frequently embarrassed by her ignorance of domestic affairs. For, whilst receipt books for elegant preparations were often seen, those connected with the ordinary, but far more useful part of household duties, were not easily procured; thus situated, she applied to persons of experience, and embodied the information collected in a book, to which, since years have matured her judgment, she has added much that is the result of her own experiments.<br /><br />Familiar, then, with the difficulties a young housekeeper encounters, when she finds herself in reality the mistress of an establishment, the Authoress offers to her young countrywomen this Work, with the belief that, by attention to its contents, many of the cares attendant on a country or city life, may be materially lessened; and hoping that the directions are such as to be understood by the most inexperienced, it is respectfully dedicated to those who feel an interest in domestic affairs.<br />Summary by the Authoress.

The first volume of Lea’s monumental work on the Inquisition of Spain, covering its origin and establishment and its relations with the state. Also included are appendices listing Tribunals, Inquisitors-General, and Spanish coinage. (Summary by Ruth Golding)

Volume 2 of Lea’s monumental work on the Spanish Inquisition. Volume 2 discusses the organizational structure of the Inquisition, methods used to financially support the Inquisition, methods used by the Inquisition to expose heresy, and numerous other topics. (Summary by JoeD)

The 3rd volume of Lea's monumental work on the Spanish Inquisition. This volume covers torture practices; the trial process; punishments; Jews, Moriscos, and Protestants; and censorship. - Summary by Sienna

The fourth and final volume of Lea's monumental work on the Spanish Inquisition. This volume discusses how the Inquisition dealt with mysticism, solicitation of illicit relationships, bigamy, theological propositions, witchcraft and sorcery, political activity, and almost every other facet of daily life. It concludes with an overarching history of the Inquisition and retrospective. (Summary by TriciaG)