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A long time Lake Orion resident is in need of a bone marrow transplant and she is asking the community for help!

A long time Lake Orion resident is in need of a bone marrow transplant and she is asking the community for help!

National Bone Marrow Program Icon

Stephanie Swanberg, Information Literacy & eLearning Librarian at Oakland University's William Beaumont School of Medicine, exclaimed that blood donations and registration on the bone marrow donor registry can help patients with diseases such as Lymphoma and other cancers be treated and even save their lives! Find more information on the Be the Match registry at https://join.bethematch.org/s/landing?language=en_US Stay up to date with our videos by subscribing to our channel: https://www.youtube.com/user/CivicCenterTV15?sub_confirmation=1. Visit our website for more information on the Megacast and Coronavirus: https://civiccentertv.com/ Follow us ⬇ ⬇ • https://www.youtube.com/user/CivicCenterTV15 • https://www.facebook.com/civiccentertv15/ • https://twitter.com/CivicCenterTV • https://www.instagram.com/civiccentertv/

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This article is from Journal of Radiation Research , volume 55 . Abstract Postnatal hematopoietic progenitor cells do not contribute to microglial homeostasis in adult mice under normal conditions. However, previous studies using whole-body irradiation and bone marrow (BM) transplantation models have shown that adult BM cells migrate into the brain tissue and differentiate into microglia (BM-derived microglia; BMDM). Here, we investigated whether cranial irradiation alone was sufficient to induce the generation of BMDM in the adult mouse brain. Transgenic mice that express green fluorescent protein (GFP) under the control of a murine stem cell virus (MSCV) promoter (MSCV-GFP mice) were used. MSCV-GFP mice express GFP in BM cells but not in the resident microglia in the brain. Therefore, these mice allowed us to detect BM-derived cells in the brain without BM reconstitution. MSCV–GFP mice, aged 8–12 weeks, received 13.0 Gy irradiation only to the cranium, and BM-derived cells in the brain were quantified at 3 and 8 weeks after irradiation. No BM-derived cells were detected in control non-irradiated MSCV-GFP mouse brains, but numerous GFP-labeled BM-derived cells were present in the brain stem, basal ganglia and cerebral cortex of the irradiated MSCV-GFP mice. These BM-derived cells were positive for Iba1, a marker for microglia, indicating that GFP-positive BM-derived cells were microglial in nature. The population of BMDM was significantly greater at 8 weeks post-irradiation than at 3 weeks post-irradiation in all brain regions examined. Our results clearly show that cranial irradiation alone is sufficient to induce the generation of BMDM in the adult mouse.

This article is from Laboratory Animal Research , volume 30 . Abstract Bone marrow (BM) has been considered as a reservoir of stem/progenitor cells which are able to differentiate into ectodermal, endodermal, and mesodermal origins in vitro as well as in vivo. Following adequate stimulation, such as granulocyte stimulating factor (G-CSF) or AMD3100, BM resident stem/progenitor cells (BMSPCs) can be mobilized to peripheral blood. Several host-related factors are known to participate in this mobilization process. In fact, a significant number of donors are resistant to G-CSF induced mobilization protocols. AMD3100 is currently used in combination with G-CSF. However, information regarding host-related factors which may influence the AMD3100 directed mobilization is extremely limited. In this study, we were to get some more knowledge on the host-related factors that affect the efficiency of AMD3100 induced mobilization by employing in vivo mobilization experiments. As a result, we found that C57BL/6J mice are more sensitive to AMD3100 but less sensitive to G-CSF which promotes the proliferation of BMSPCs. We excluded S1P as one of the host related factor which influences AMD3100 directed mobilization because pre-treatment of S1P receptor antagonist FTY720 did not inhibit BMSPC mobilization. Further in vitro experiments revealed that BALB/c mice, compared to C57BL/6J mice, have less BMSPCs which migrate in response to host related factors such as sphingosine-1-phosphate (S1P) and to CXCL12. We conclude that AMD3100-directed mobilization depends on the number of BMSPCs rather than on the host-related factors. These results suggest that the combination of AMD3100 and G-CSF is co-operative and is optimal for the mobilization of BMSPCs.

This article is from Neural Regeneration Research , volume 8 . Abstract In this study, a combination of growth factors was used to induce bone marrow mesenchymal stem cells differentiation into neuron-like cells, in a broader attempt to observe the role of thrombospondin 1 in synapse formation. Results showed that there was no significant difference in the differentiation rate of neuron-like cells between bone marrow mesenchymal stem cells with thrombospondin induction and those without. However, the cell shape was more complex and the neurites were dendritic, with unipolar, bipolar or multipolar morphologies, after induction with thrombospondin 1. The induced cells were similar in morphology to normal neurites. Immunohistochemical staining showed that the number of positive cells for postsynaptic density protein 95 and synaptophysin 1 protein was significantly increased after induction with thrombospondin 1. These findings indicate that thrombospondin 1 promotes synapse formation in neuron-like cells that are differentiated from bone marrow mesenchymal stem cells.

This article is from BMC Oral Health , volume 14 . Abstract Background: Debridement and disinfection of the root canal system is a crucial step in endodontic procedures. The effectiveness of irrigation relies on both the mechanical flushing action and the ability of irrigants to dissolve tissue and kill bacteria. The objective of the present study is to evaluate and compare the cytotoxicity of QMix™ root canal irrigating solution on immortalized human bone marrow mesenchymal stem cells (hTERT-MSC-C1) and to compare it with that of sodium hypochlorite (NaOCl). Methods: Immortalized human bone marrow mesenchymal stem cells (hTERT-MSCs) were exposed to QMix™ and NaOCl. Cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and alamarBlue assays. The cell morphology was studied after two hours of exposure to QMix™ and NaOCl. Scanning electron microscopy (SEM) analyses were performed after 2- and 4-hour incubation periods. Finally, ethidium bromide/acridine orange (EB/AO) fluorescent stain was applied to the cells in the 8-chamber slides after they were incubated with the testing agents for 2 hours to detect live and dead cells. The observations were tabulated and analyzed statistically. Results: QMix™ exposure resulted in a significantly higher percentage of cell viability than NaOCl in the MTT and alamarBlue assays at three time points compared to the control. The SEM analysis demonstrated minimal morphological changes associated with cells that were exposed to the QMix™ solution, with little shrinkage and fragmentation of the cell wall. The live/dead analysis showed that the number of live cells after exposure to QMix™ was similar to that of the untreated control. No cell structure could be observed with the NaOCl group, indicating cell lysis. Conclusion: Both the QMix™ and NaOCl solutions were toxic to human bone marrow MSCs. Each solution might have induced cell death in a different way as evidenced in the cell viability, SEM and fluorescent studies. The slower cell death induced by QMix™ might therefore be less aggressive and more acceptable to living tissues.

This article is from South Asian Journal of Cancer , volume 2 . Abstract Background:: In many South Asian countries there is shortage of centers providing care for pediatric malignancies. This report describes the experience of the Cure2Children Foundation (C2C) in supporting, both financially and professionally, the startup of two bone marrow transplant (BMT) centers, one in Pakistan and one in India, for the cure of transfusion-dependent thalassemia. Even though transplantation is generally considered as a more complex and advanced step relatively to basic pediatric cancer care, the authors argue that BMT for low-risk thalassemia patients with a matched sibling is a relatively simple procedure amenable to focused training. Materials and Methods:: Since 2008 the C2C, an Italian Nongovernmental Organization (NGO), has supported a BMT network in Pakistan. The primary aim of this project was to assess feasibility, outcomes, and costs of matched-related BMT for thalassemia in young low-risk children employing a well established and quite tolerable strategy employed in Italy. This initiative relied primarily on focused training and task-shift strategies within a structured cooperation program. The initial success of that strategy led to its replication in India with 100 total BMTs performed over the past 4 years, 91 of which were for thalassemia major. Results:: Low-risk matched-related BMT in children younger than 5 years could deliver a 92% thalassemia-free survival with 100% performance score and no extensive chronic graft versus host disease (GVHD), for an average cost of 10,000 USD per BMT. Within an existing hospital facility, 50,000 USD were sufficient to renovate and fully equip a 2-3 bedded start up BMT unit capable of performing safe low-risk compatible marrow transplantation. Conclusions:: In low resource settings matched-related low-risk BMT for thalassemia can be performed with outcomes comparable to richer countries and with a fraction of the costs. Within structured and intensive cooperation, good outcomes can be obtained from the very beginning. This observation may have important implications to increase access to cure for both nonmalignant and malignant.

This article is from PLoS ONE , volume 9 . Abstract Asherman's Syndrome is characterized by intrauterine adhesions or fibrosis resulting as a consequence of damage to the basal layer of endometrium and is associated with infertility due to loss of normal endometrium. We have previously shown that bone marrow derived stem cells (BMDSCs) engraft the endometrium in mice and humans and Ischemia/reperfusion injury of uterus promoted BMDSCs migration to the endometrium; however, the role of BMDSCs in Asherman's syndrome has not been characterized. Here a murine model of Asherman's syndrome was created by traumatizing the uterus. We evaluate stem cell recruitment and pregnancy after BMDSCs transplantation in a model of Asherman's syndrome. In the Asheman's syndrome model, after BMDSC transplant, the Y chromosome bearing CD45-cells represented less than 0.1% of total endometrial cells. Twice the number of Y+CD45- cells was identified in the damaged uterus compared to the uninjured controls. There was no significant difference between the damaged and undamaged uterine horns in mice that received injury to a single horn. In the BMDSC transplant group, 9 of the 10 mice conceived, while only 3 of 10 in the non-transplanted group conceived (Chi-Square p = 0.0225); all mice in an uninjured control group conceived. The time to conception and mean litter size were not different between groups. Taken together, BMDSCs are recruited to endometrium in response to injury. Fertility improves after BMDSC transplant in Asherman's Syndrome mice, demonstrating a functional role for these cells in uterine repair. BMDSC transplantation is a potential novel treatment for Asherman's Syndrome and may also be useful to prevent Asherman's syndrome after uterine injury.

This article is from Journal of Translational Medicine , volume 12 . Abstract Backgroud: Bone tissue engineering is a new approach for the repair of orbital defects. The aim of the present study was to explore the feasibility of tissue-engineered bone constructed using bone marrow stromal cells (BMSCs) that were rapidly isolated and concentrated from bone marrow (BM) by the red cell lysis method, then combined with β-tricalcium phosphate (β-TCP) to create grafts used to restore orbital bone defects in canines. Methods: In the experimental group, grafts were constructed using BMSCs obtained by red cell lysis from 20 ml bone marrow, combined with β-TCP and BM via the custom-made stem cell-scaffold device, then used to repair 10 mm diameter medial orbital wall bony defects in canines. Results were compared with those in groups grafted with BM/β-TCP or β-TCP alone, or with defects left untreated as controls. The enrichment of BMSCs and nucleated cells (NCs) in the graft was calculated from the number in untreated bone marrow and in suspensions after red cell lysis. Spiral computed tomography (CT) scans were performed 1, 4, 12 and 24 weeks after implantation in all groups. Gross examination, micro-CT and histological measurements were performed 24 weeks after surgery. The results were analyzed to evaluate the efficacy of bone repair. Results: The number of NCs and of colony-forming units within the scaffolds were increased 54.8 times and 53.4 times, respectively, compared with untreated bone marrow. In the BMSC-BM/β-TCP group, CT examination revealed that the scaffolds were gradually absorbed and the bony defects were restored. Micro-CT and histological examination confirmed that the implantations led to good repair of the defects, with 6 out 8 orbital defects completely restored in the experimental group, while by contrast, the grafts in the control groups did not fully repair the bony defects, a difference which was statistically significant (p 

This article is from Journal of Translational Medicine , volume 12 . Abstract Backgroud: Bone tissue engineering is a new approach for the repair of orbital defects. The aim of the present study was to explore the feasibility of tissue-engineered bone constructed using bone marrow stromal cells (BMSCs) that were rapidly isolated and concentrated from bone marrow (BM) by the red cell lysis method, then combined with β-tricalcium phosphate (β-TCP) to create grafts used to restore orbital bone defects in canines. Methods: In the experimental group, grafts were constructed using BMSCs obtained by red cell lysis from 20 ml bone marrow, combined with β-TCP and BM via the custom-made stem cell-scaffold device, then used to repair 10 mm diameter medial orbital wall bony defects in canines. Results were compared with those in groups grafted with BM/β-TCP or β-TCP alone, or with defects left untreated as controls. The enrichment of BMSCs and nucleated cells (NCs) in the graft was calculated from the number in untreated bone marrow and in suspensions after red cell lysis. Spiral computed tomography (CT) scans were performed 1, 4, 12 and 24 weeks after implantation in all groups. Gross examination, micro-CT and histological measurements were performed 24 weeks after surgery. The results were analyzed to evaluate the efficacy of bone repair. Results: The number of NCs and of colony-forming units within the scaffolds were increased 54.8 times and 53.4 times, respectively, compared with untreated bone marrow. In the BMSC-BM/β-TCP group, CT examination revealed that the scaffolds were gradually absorbed and the bony defects were restored. Micro-CT and histological examination confirmed that the implantations led to good repair of the defects, with 6 out 8 orbital defects completely restored in the experimental group, while by contrast, the grafts in the control groups did not fully repair the bony defects, a difference which was statistically significant (p 

Book Source: Digital Library of India Item 2015.549269 dc.contributor.author: Talbott, G. D. dc.date.accessioned: 2015-10-14T21:04:02Z dc.date.available: 2015-10-14T21:04:02Z dc.date.copyright: 1957 dc.date.digitalpublicationdate: 2011/02 dc.date.citation: 1957 dc.identifier.barcode: 04990010029315 dc.identifier.origpath: /data8/upload/0226/597 dc.identifier.copyno: 1 dc.identifier.uri: http://www.new.dli.ernet.in/handle/2015/549269 dc.description.scannerno: Banasthali University dc.description.scanningcentre: C-DAK, Kolkata dc.description.main: 1 dc.description.tagged: 0 dc.description.totalpages: 66 dc.format.mimetype: application/pdf dc.language.iso: Sanskrit dc.publisher.digitalrepublisher: Digital Library of India dc.publisher: New York, Grune & Stratton dc.rights: Copyright permitted dc.source.library: Dr. Robert Heiling Library, S.m.s.medical College, Jaipur dc.subject.classification: Medical dc.title: Blood And Bone Marrow Patterns dc.type: Print - Paper dc.type: Book

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This article is from Journal of Translational Medicine , volume 10 . Abstract Background: Endometrial regenerative cells (ERC) and bone marrow stromal cells (BMSC) are being used in clinical trials. While they have been reported to have similar characteristics, they have not been directly compared. Methods: We compared micro RNA (miRNA) and gene expression profiles, soluble cytokine and growth factor levels and ability to inhibit ongoing mixed leukocyte reaction (MLR) of ERC and BMSC each derived from 6 healthy subjects. Results: ERC and BMSC miRNA and gene expression profiles were similar, but not identical; more differences were noted in the expression of genes than in miRNAs. Genes overexpressed in ERCs were more likely to be in immune and inflammation pathways and those overexpressed in BMSCs were more likely to be in stem cell and cancer signaling pathways. In addition, the levels of IL-8 and ICAM-1 were greater in ERC supernatants while the levels of HGF, VEGF, IL-6, CXCL12, TGFB1 and TGFB2 were greater in BMSC supernatants. Additionally, ERC demonstrated greater inhibition of the proliferation of mixed leukocyte cultures. Conclusions: These results suggest that the in vivo effects of ERC and BMSC may differ. Multiple properties of stromal cells are responsible for their in vivo effectiveness and ERC may be more effective for some of the clinical applications and BMSC for others. Studies in animal models or clinical trials will be required to more fully characterize the differences between ERC and BMSC.

This article is from PLoS ONE , volume 9 . Abstract CD73 catalyzes the conversion of extracellular nucleosides to adenosine, modulating inflammatory and T cell responses. Elevated expression of CD73 marks subpopulations of murine memory B cells (MBC), but its role in memory development or function is unknown. Here, we demonstrate that CD73 is progressively upregulated on germinal center (GC) B cells following immunization, is expressed at even higher levels among T follicular helper cells, but is absent among plasma cells (PC) and plasmablasts (PB). We analyzed the T-dependent B cell response in CD73 knockout mice (CD73KO). During the early response, CD73KO and wild type (WT) mice formed GCs, MBCs and splenic PBs and PCs similarly, and MBCs functioned similarly in the early secondary response. Late in the primary response, however, bone marrow (BM) PCs were markedly decreased in CD73KO animals. Tracking this phenotype, we found that CD73 expression was required on BM-derived cells for optimal BM PC responses. However, deletion of CD73 from either B or T lymphocytes alone did not recapitulate the phenotype. This suggests that CD73 expression is sufficient on either cell type, consistent with its function as an ectoenzyme. Together, these findings suggest that CD73-dependent adenosine signaling is prominent in the mature GC and required for establishment of the long-lived PC compartment, thus identifying a novel role for CD73 in humoral immunity.

This article is from PLoS ONE , volume 9 . Abstract CD73 catalyzes the conversion of extracellular nucleosides to adenosine, modulating inflammatory and T cell responses. Elevated expression of CD73 marks subpopulations of murine memory B cells (MBC), but its role in memory development or function is unknown. Here, we demonstrate that CD73 is progressively upregulated on germinal center (GC) B cells following immunization, is expressed at even higher levels among T follicular helper cells, but is absent among plasma cells (PC) and plasmablasts (PB). We analyzed the T-dependent B cell response in CD73 knockout mice (CD73KO). During the early response, CD73KO and wild type (WT) mice formed GCs, MBCs and splenic PBs and PCs similarly, and MBCs functioned similarly in the early secondary response. Late in the primary response, however, bone marrow (BM) PCs were markedly decreased in CD73KO animals. Tracking this phenotype, we found that CD73 expression was required on BM-derived cells for optimal BM PC responses. However, deletion of CD73 from either B or T lymphocytes alone did not recapitulate the phenotype. This suggests that CD73 expression is sufficient on either cell type, consistent with its function as an ectoenzyme. Together, these findings suggest that CD73-dependent adenosine signaling is prominent in the mature GC and required for establishment of the long-lived PC compartment, thus identifying a novel role for CD73 in humoral immunity.

Linda Leatherdale is the mother of Skye, her daughter who was near death before a bone marrow match was discovered in the U.S. Skye who was 21 at the time has not only survived beyond five years, she is married and looking forward to a full life.For more, including Roy's conversation with Toby Boulet, listen to the Roy Green Show Podcast. And subscribe to the show wherever you find the podcasts that matter to you, including Apple Podcasts and Google Podcasts.Guest: Linda Leatherdale, Vice President of Cambria Canada, Former Money editor with the Toronto SunLearn more about your ad choices. Visit megaphone.fm/adchoices

Stephanie Swanberg, Information Literacy & eLearning Librarian at Oakland University's William Beaumont School of Medicine, explained that the boen marrow drive will occur entirely virtually this year, with entrants registering via a website and with Oakland competing with other universities and colleges to have the most donors sign up! Stay up to date with our videos by subscribing to our channel: https://www.youtube.com/user/CivicCenterTV15?sub_confirmation=1. Visit our website for more information on the Megacast and Coronavirus: https://civiccentertv.com/ Follow us ⬇ ⬇ • https://www.youtube.com/user/CivicCenterTV15 • https://www.facebook.com/civiccentertv15/ • https://twitter.com/CivicCenterTV • https://www.instagram.com/civiccentertv/

Stephanie Swanberg, Information Literacy & eLearning Librarian at Oakland University's William Beaumont School of Medicine, explained that the boen marrow drive will occur entirely virtually this year, with entrants registering via a website and with Oakland competing with other universities and colleges to have the most donors sign up! Stay up to date with our videos by subscribing to our channel: https://www.youtube.com/user/CivicCenterTV15?sub_confirmation=1. Visit our website for more information on the Megacast and Coronavirus: https://civiccentertv.com/ Follow us ⬇ ⬇ • https://www.youtube.com/user/CivicCenterTV15 • https://www.facebook.com/civiccentertv15/ • https://twitter.com/CivicCenterTV • https://www.instagram.com/civiccentertv/

This protocol has been accruing subjects appropriately and will close to accrual soon. During this report year 13subjects were consented; 13 were screened; and, 1 was deemed a 'screen failure'. Two subjects (11-002 and 12-1) were withdrawn from the study; one by the local Pl (the center could not obtain records needed for screening from the referring institution) and one by the central site Pl (the subject was reported to be on Zenpep for gastrointestinal absorption issues and there are no data on the absorption of Leucine in this setting). There was one severe adverse event reported in subject 02-005 the subject had an anaphylactic reaction to a blood transfusion (33 minutes from the start of the transfusion) and was no deemed associated with the study drug. The subject was admitted for observation and was discharged the following day without any further issues.

Severe congenital neutropenia (SCN) is an inherited bone marrow failure syndrome most often resulting from autosomal dominant or de novo transmission of heterozygous mutations in the gene, ELANE, encoding neutrophil elastase. Other causes of SCN include autosomal recessive inheritance of HAX1 mutations. The purpose of this research is to understand how mutations in both ELANE and HAX1 lead to neutropenia, in order to gain further understanding into normal homeostatic regulation of granulopoiesis and how it is disrupted in a variety of bone marrow failure syndromes. Based on identification of a new class of mutations in ELANE that disrupt the translational start site and recent findings that HAX1 may be an RNA-binding protein, we originally hypothesized, and have now shown in patient-derived induced pluripotent stem cells (iPSC), that ELANE can encode amino-terminally truncated polypeptides initiating from internal translational start sites. In scope, we have proposed a series of experiments to be performed in cell culture systems to identify RNA sequences bound by HAX1, to determine if ELANE mutations influence HAX1 binding to ELANE mRNA and influence use of alternate translational start sites, and to biochemically characterize amino-terminally truncated neutrophil elastase. In the year since our last report, significant progress has been made in using new tools to model granulopoiesis, including generation of patient-derived iPSC and CRISPR-Cas9 genome-editing technology to introduce site specific repair or mutation into the ELANE locus, allowing for more relevant modeling of ELANE and HAX1 interactions in vitro.

Severe congenital neutropenia (SCN) is an inherited bone marrow failure syndrome most often resulting from autosomal dominant or de novo transmission of heterozygous mutations in the gene, ELANE, encoding neutrophil elastase. Other causes of SCN include autosomal recessive inheritance of HAX1 mutations. The purpose of this research is to understand how mutations in both ELANE and HAX1 lead to neutropenia, in order to gain further understanding into normal homeostatic regulation of granulopoiesis and how it is disrupted in a variety of bone marrow failure syndromes. Based on identification of a new class of mutations in ELANE that disrupt the translational start site and recent findings that HAX1 may be an RNA-binding protein, we originally hypothesized, and have now shown in patient-derived induced pluripotent stem cells (iPSC), that ELANE can encode amino-terminally truncated polypeptides initiating from internal translational start sites. In scope, we have proposed a series of experiments to be performed in cell culture systems to identify RNA sequences bound by HAX1, to determine if ELANE mutations influence HAX1 binding to ELANE mRNA and influence use of alternate translational start sites, and to biochemically characterize amino-terminally truncated neutrophil elastase. In the year since our last report, significant progress has been made in using new tools to model granulopoiesis, including generation of patient-derived iPSC and CRISPR-Cas9 genome-editing technology to introduce site specific repair or mutation into the ELANE locus, allowing for more relevant modeling of ELANE and HAX1 interactions in vitro.

A University of Alabama student organization needs your help to save lives. Be the Match has one goal: adding people to the National Bone Marrow Registry.   Getting on the registry is simple. All it requires is some paperwork and a swab on the inside of the mouth. Then the swab to a lab, where it will hopefully be matched with a cancer patient in need of a bone marrow transplant.

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Kemik iliğinin seröz yağ atrofisi ( jelatinöz kemik iliği transformasyonu) nadir görülen patogenezi bilinmeyen bir antitedir. İlk ola-rak 1898 yılında tanımlanmıştır. Seröz atrofiye AIDS (Acquired Immuno Deficiency Syndrome), alkolizm, anoreksia nevroza, ka-şeksi, karsinom veya lenfoma eşlik edebilmektedir [1]…

xii, 212 pages : 29 cm

xii, 212 pages : 29 cm

xii, 212 pages : 29 cm

xii, 212 pages : 29 cm

Our earlier results showed that the presence of cytokeratin positive epithelial cells in the bone marrow is an independent predictor of worse prognosis in human breast cancer. The biological origin and the cellular and molecular characteristics of these cells in not known and was the objective of the present proposal. We have characterized a set of 47 primary breast cancers with respect to expression of mutant p53 and HSP27. Our results are consistent with the findings that expression of mutant p53 is associated with metastatic spread be it occult as found in bone marrow or positive lymph nodes. 57% of pS3 eve tumors were associated with bone marrow micrometastasis (BMM) as compared to 15% of p53 -ve tumors with BMM positive. We have developed a novel culture technique that can be adapted to separate the epithelial cancer cells in the bone marrow. This will allow us to fully characterize these cells at the cellular and molecular level and define their origin, thus fulfilling the major objective of this proposal.

Circulating Mesenchymal Stem Cells (MSCs) originating from the bone marrow have the ability to differentiate into cells of the mesoderm lineage and an innate tropism for tumor tissue in response to the inflammatory microenvironment present in malignant lesions. MSCs have been detected in the perivascular space of many tumors, including those of the prostate, and have been shown to be a critical element in oncogenic progression. MSCs are inherently non-immunogenic, which prevents allogeneic MSCs from being rejected by normal host defense mechanisms. This immune-privileged status, together with their oncotropic properties, makes possible the infusion of allogeneic MSCs into patients for therapeutic purposes, such as the delivery of cytotoxic agents to sites of primary and metastatic prostate cancer. PRX302 is a PSA-activated aerolysin-based protoxin that forms membrane pores and leads to necrosis by a proliferation-independent mechanism at low picomolar concentrations. Importantly, PRX302 binds with low nanomolar affinity to GPI-anchor proteins, which are highly expressed on the surface of all mammalian cells. Therefore, MSCs can be genetically manipulated to express the PRX302 transgene endogenously from a safe harbor locus. Based upon this rationale we hypothesize that human bone marrow-derived mesenchymal stem cells (hMSCs) can be used as a cell-based targeting vehicle to selectively deliver therapeutic agents, such as PRX302, to primary and metastatic sites of prostate cancer, and thus spare host toxicity.

The specific objectives of this study were (1) to determine to what extent cell turnover (reproduction) effects the difference in radiation sensitivity of the individual cell types, and (2) to investigate the relationship which exists among the cell types during the damage and recovery period. Six hundred and seventy-two rats were exposed to either of the two radiation sources and sacrificed at periodic intervals following exposure for the purpose of determining the change in numbers of cells in the marrow. The results indicate that the difference in the response of the individual types of cells is not directly related to their turnover rate. The turnover rate for the myelocytic cells is greater than the turnover rate for the erythrocytic cells, yet the erythrocytic precursor cells are more sensitive to radiation than the myelocytic precursor cells. The results also show that erythrocytic cells recover before myelocytic or lymphocytic cells. This recovery indicates that lymphocytic cells probably could not give rise to the other two cell types (function as a stem cell) through a process of differentiation or dedifferentiation. However, it may be possible that the lymphocytic cells could function as trephocytes.

Autologous Bone Marrow Transplantation (ABMT) management involves providing complex care for severely ill and immunocompromised patients. The ability to identify% biodemographic, psychological and/or social factors which contribute to symptom development would be instrumental in the medical and nursing management of patients undergoing ABMT. This study examined the relationship between health perception and symptom severity in post ABMT patients. A secondary analysis was performed on data from a convenience sample of 20 patients. Health perceptions were measured by Ware's Health Perception Questionnaire. The severity of pain, nausea, vomiting and depression were measured using questions from the European Organization Research and Treatment Quality of Life Questionnaire and Bush Late Complications of BMT Module. Approximately 65% of the patients in this study experienced pain, 30 % experienced nausea, and 35 % experienced depression post ABMT. Vomiting was not experienced by any of the patients. Pearson's product moment correlation was calculated and showed the relationship between the total scores for health perception and symptom severity was significant ( r = -.463, p = 0.05), and revealed that the more severe the patient's symptoms were the lower their health perception was.

National Bone Marrow Transplant Link Executive Director, Peggy Burkhard talks about the organization’s efforts to raise awareness for bone marrow and stem cell donations, as well as provide information on services to those in need of these procedures or who are recovering from these procedures! Stay up to date with our videos by subscribing to our channel: https://www.youtube.com/user/CivicCenterTV15?sub_confirmation=1. Visit our website for more information on the Megacast and The Splash: https://civiccentertv.com/ Follow us ⬇ ⬇ • https://www.youtube.com/user/CivicCenterTV15 • https://www.facebook.com/civiccentertv15/ • https://twitter.com/CivicCenterTV • https://www.instagram.com/civiccentertv/

Take your taste buds on a journey they won't soon forget with Niku X's Roasted Wagyu Bone Marrow, a dish that is as flavorful as it is visually stunning. As we pour an overproof bourbon, rich in caramel and spice, igniting it adds another level of heat to the bone marrow allowing all the flavors and aromas come to life, making each bite an experience in itself. Enjoy the bone marrow with a slice of Japanese Milk Bread Toast and let the rich, buttery texture melt in your mouth. Come and discover this culinary gem at Niku X today! . . .  #a5wagyu #yakiniku #japanesefood #wagyubeef #japanesewagyu #finedining #wagyujournal #lafood #lafoodie #laeats #losangeles #eaterla #losangelesfood #lafoodies #yelpla #dinela  #la #losangelesfoodie #infatuationla #losangeleseats #eeeeeats  #laeater #ocfood #bestfoodla #latimesfood  #dtla #nikux Source: https://www.tiktok.com/@7086544349480502274/video/7223201107164532011 Uploader: nikuxla

This article is from Chinese Journal of Cancer , volume 33 . Abstract This perspective article highlights the leukocyte-cancer cell hybrid theory as a mechanism for cancer metastasis. Beginning from the first proposal of the theory more than a century ago and continuing today with the first proof for this theory in a human cancer, the hybrid theory offers a unifying explanation for metastasis. In this scenario, leukocyte fusion with a cancer cell is a secondary disease superimposed upon the early tumor, giving birth to a new, malignant cell with a leukocyte-cancer cell hybrid epigenome.

This article is from Journal of Tissue Engineering , volume 2 . Abstract Low-molecular-weight heparin/protamine microparticles (LH/P MPs) serve as carriers for controlled release of heparin-binding cytokines. LH/P MPs were stably coated onto plastic surfaces by drying. The purpose of this study is to evaluate a culture method for selective expansion of CD34+ cells using LH/P MPs as cytokine-binding matrix. Ficoll-purified mouse bone marrow cells (mouse FP-BMCs) containing CD34+ cells were cultured on LH/P MP-coated plates in the presence of stem cell factor (SCF), thrombopoietin (Tpo), and Flt-3 ligand (Flt-3) in hematopoietic progenitor growth medium (HPGM) supplemented with 4% heat-inactivated fetal bovine serum (FBS). After 8 days of culture, the total cell count increased 4.6-fold, and flow cytometry analyses revealed that 23.8% of the initial cells and 57.4% of the expanded cells were CD34 positive. Therefore, CD34+ cells were estimated to have increased 11.0-fold. In contrast, cultured CD34+ cells on uncoated tissue culture plates increased 5.8-fold in an identical medium.

This article is from Experimental and Therapeutic Medicine , volume 4 . Abstract The present study aimed to observe the effect of rat bone marrow mesenchymal stem cells (MSCs) in vitro on hepatic stellate cell (HSC) RhoA signaling factors and the expression of the cell cycle regulators P27 and cyclin D1. Rat HSC-T6 and fibroblast cells were divided into control, negative control and MSC experimental groups. The cell proliferation rate was examined using the WST8 assay. The cell cycle was analyzed using flow cytometry. RT-PCR and western blot analysis were used to examine cyclin in D1 (cyclin D1), RhoA and P27 mRNA and protein expression in HSCs. After 12 h of co-culture, transition of the MSCs from the G0/G1 to S phase was blocked by HSCs. In the MSC experimental group, the RhoA mRNA and RhoA protein expression showed a decreasing trend with time, which was statistically significant compared with that in the control and negative control groups. MSC P27 protein expression showed an increasing trend with time. RhoA and P27 expression were significantly negatively correlated. After 24 h of co-culture, MSCs inhibited cyclin D1 expression. The difference was statistically significant in the experimental and control groups as well as in the negative control group (P

Source: USC Shoah Foundation Jewish Survivor Magda Klein Testimony https://www.youtube.com/watch?v=R0qSlgDWTvA

Includes bibliographies and index

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Task 1 : Evaluate optimal short term storage parameters for stimulated and unstimulated leukapheresis (donor lymphocytes) and bone marrow products, including the type of storage media and the cell concentration, in addition to temperature and duration of storage before processing or infusion. Task 2: The NMDP has developed an algorithm that predicts' high resolution HLA typing results on donor samples that exist in the Registry with only low or intermediate results reported. Perform validation of the NMDP algorithm by selecting donors randomly from our Registry that have low or intermediate DRB1 typing results and using the algorithm to predict the high resolution results and test the ability of the algorithm to predict KIR ligand mismatching in the absence of existing HLA-C locus results.

Acute graft-versus-host disease (GVHD) remains a significant cause of morbidity and mortality following allogeneic bone marrow transplantation (BMT), with conventional immunosuppressive therapies offering limited efficacy and considerable adverse effects. In recent years, nanoparticle-based drug delivery systems and immunomodulatory strategies have emerged as promising targeted approaches in preclinical transplantation models. However, no systematic review has yet comprehensively evaluated their effectiveness in reducing acute GVHD in experimental murine models. This systematic review aims to assess the efficacy of various nanoparticle-based interventions—including nanoencapsulation of immune cells and targeted delivery of immunosuppressive agents—in mitigating the severity of acute GVHD compared to standard immunosuppression in well-established murine BMT models. We will systematically search databases for preclinical randomized controlled studies involving nanoparticle-mediated therapies administered via intravenous or intraperitoneal routes in experimental murine models of BMT with induced acute GVHD. The primary outcome will be the severity of acute GVHD, while secondary outcomes include graft survival, graft-versus-leukemia (GVL) activity, and other BMT-related survival metrics. This review will address a critical gap in transplantation research by synthesizing evidence on the use of nanoparticle-based immunomodulation in BMT, providing a foundation for future translational studies and clinical applications.

Note this registration has been withdrawn: The pathophysiology of idiopathic bone marrow edema syndrome is largely unknown. Furthermore idiopathic bone marrow edema syndrome is an underdiagnosed disease with limited evidence about diagnostics and therapy. Therefore, the aim of this scoping review was to systematically combine the available evidence about epidemiology, etiology, diagnostics, and therapy, as well as to identify knowledge gaps for further research.

Note this registration has been withdrawn: The pathophysiology of idiopathic bone marrow edema syndrome is largely unknown. Furthermore idiopathic bone marrow edema syndrome is an underdiagnosed disease with limited evidence about diagnostics and therapy. Therefore, the aim of this scoping review was to systematically combine the available evidence about epidemiology, etiology, diagnostics, and therapy, as well as to identify knowledge gaps for further research.

Opioids are often used to facilitate sedation in pediatric patients receiving bone marrow biopsies and intrathecal chemotherapy injections (Abdolkarimi et al. 2016). Few studies have been undertaken to compare opioid-containing sedation with opioid-free sedation for bone marrow biopsy and intrathecal chemotherapy administration, particularly in children.

This article is from Radiation Oncology (London, England) , volume 7 . Abstract Background: Ionizing radiation-induced hematopoietic injury could occur either due to accidental exposure or due to diagnostic and therapeutic interventions. Currently there is no approved drug to mitigate radiation toxicity in hematopoietic cells. This study investigates the potential of ON 01210.Na, a chlorobenzylsulfone derivative, in ameliorating radiation-induced hematopoietic toxicity when administered after exposure to radiation. We also investigate the molecular mechanisms underlying this activity. Methods: Male C3H/HeN mice (n = 5 mice per group; 6-8 weeks old) were exposed to a sub-lethal dose (5 Gy) of γ radiation using a 137Cs source at a dose rate of 0.77 Gy/min. Two doses of ON 01210.Na (500 mg/kg body weight) were administered subcutaneously at 24 h and 36 h after radiation exposure. Mitigation of hematopoietic toxicity by ON 01210.Na was investigated by peripheral white blood cell (WBC) and platelet counts at 3, 7, 21, and 28 d after radiation exposure. Granulocyte macrophage colony forming unit (GM-CFU) assay was done using isolated bone marrow cells, and terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) was performed on bone marrow sections at 7 d post-exposure. The DNA damage response pathway involving ataxia telangiectasia mutated (ATM) and p53 was investigated by Western blot in bone marrow cells at 7 d post-exposure. Results: Compared to the vehicle, ON 01210.Na treated mice showed accelerated recovery of peripheral WBC and platelet counts. Post-irradiation treatment of mice with ON 01210.Na also resulted in higher GM-CFU counts. The mitigation effects were accompanied by attenuation of ATM-p53-dependent DNA damage response in the bone marrow cells of ON 01210.Na treated mice. Both phospho-ATM and phospho-p53 were significantly lower in the bone marrow cells of ON 01210.Na treated than in vehicle treated mice. Furthermore, the Bcl2:Bax ratio was higher in the drug treated mice than the vehicle treated groups. Conclusions: ON 01210.Na treatment significantly mitigated the hematopoietic toxicity induced by a sub-lethal radiation dose. Mechanistically, attenuation of ATM-p53 mediated DNA damage response by ON 01210.Na is contributing to the mitigation of radiation-induced hematopoietic toxicity.