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"DTIC ADA398169: The Functional Role(s) Of Neurofibromin During Neural Crest Cell Development" and the language of the book is English.


“DTIC ADA398169: The Functional Role(s) Of Neurofibromin During Neural Crest Cell Development” Metadata:

  • Title: ➤  DTIC ADA398169: The Functional Role(s) Of Neurofibromin During Neural Crest Cell Development
  • Author: ➤  
  • Language: English

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  • Internet Archive ID: DTIC_ADA398169

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"DTIC ADA398169: The Functional Role(s) Of Neurofibromin During Neural Crest Cell Development" Description:

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The purpose of the studies to date have been to A) produce and maintain a colony of heterozygote neurofibromin (Nf(sup +/-) in order to produce timed-pregnant neurofibromin knockout (Nf(sup -/-)) embryos. (B) assess the ability of in vitro and in situ studies in order to quantitate the survival, proliferation, migration and differentiation of neural crest cells derived from wild type (Nf(sup +/+)), Nf(sup +/-) and Nf(sup -/-) embryos. To achieve the aim in A, I have mated Nf(sup +/-) male mice with wild type mice to generate a colony of Nf(sup +/-) mice. This has proven more effective than directly mating male/female Nf(sup +/-) mice because Nf(sup +/-) have a greater tendency to cannibalize their litters versus wild-type females, With regards to B) I have firstly found it necessary to pair breeding mice during the day during a precise time window in order to accurately obtain embryonic day 9 (EB) time pregnant embryos. This is due to the narrow/transient time window of early NCC development in mice. Secondly, preliminary experiments strongly suggest that it is not feasible to the analysis neural crest development derived from individual embryos using a tissue culture paradigm but rather to use a in system whereby the NCC are analyzed directly in the embryo (in situ).

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  • Source: Internet Archive
  • Internet Archive Link: Archive.org page
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  • Number of Files: 17
  • Number of Available Files: 17
  • Added Date: 2018-05-05 20:38:07
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